1. Topic
  2. 请注意
  3. 新产品
  4. 有对应的SuperMix
  5. 模板制备
    1. 基因组DNA提取
      1. 柱式
        1. EasyPure Genomic DNA Kit
        2. EasyPure Plant Genomic DNA Kit
        3. EasyPure Blood Genomic DNA Kit
      2. 手提
        1. BloodZol
        2. PlantZol
    2. RNA提取
      1. 样品保存
        1. RNAhold
      2. 柱式
        1. EasyPure RNA Kit
        2. EasyPure Viral DNA/ RNA Kit(Carrier RNA)
        3. EasyPure Plant RNA Kit
      3. 手提
        1. TransZol
        2. TransZol Up
        3. TransZol Plant
  6. PCR产物制备
    1. PCR(基因组-PCR产物)
      1. Taq
        1. TransFast Taq DNA Polymerase
        2. EasyTaq DNA Polymerase(2×SuperMix)
        3. TransTaq-T DNA Polymerase(2×SuperMix)
        4. TransTaq DNA Polymerase High Fidelity(HiFi)(2×SuperMix)
          1. GC Enhancer(酶用)
        5. TransStart TopTaq DNA Polymerase
      2. Pfu
        1. TransStart FastPfu DNA Polymerase (2×SuperMix)
          1. PCR Stimulant(酶用)
        2. EasyPfu DNA Polymerase (2×SuperMix)
      3. SuperMix
        1. 2×Trans Blood PCR SuperMix(-dye )
    2. RT-PCR(RNA-PCR产物)
      1. Reverse Transcriptase(EasyScript、TransScript、TransScriptII)
      2. First-Strand cDNA Synthesis SuperMix
      3. TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix(用于qPCR)
      4. Two-Step RT-PCR SuperMix
      5. One-Step RT-PCR SuperMix
      6. Rinbonuclease Inhibitor
      7. 需另外购买PCR酶,进行cDNA-PCR产物的反应
  7. PCR产物纯化及检测
    1. 产物纯化
      1. EasyPure PCR Purification Kit(直接纯化)
      2. EasyPure Quick Gel Extraction Kit(胶回收纯化)
    2. 电泳检测
      1. 染料(GelStain)替代EB
      2. DNA分子量标准
        1. DNA Marker
          1. Topic
          2. Trans2K、Trans2K Plus、Trans2K Plus II
          3. Trans4K、Trans5K 、Trans8K、Trans15K
          4. Trans DNA Marker I
          5. Trans DNA Marker II
        2. DNA Ladder
          1. Topic
          2. 1Kb DNA Ladder、1Kb Plus DNA Ladder
          3. 100bp、100bp Plus 、100bp Plus II
  8. 克隆载体构建
    1. 1 连接
      1. T4 DNA Ligase(片段和载体客户自己提供)
      2. 克隆载体
        1. Topic
        2. pEASY-T1 Cloning Kit
        3. pEASY-T1 Simple Cloning Kit
        4. pEASY-T3 Cloning Kit
        5. pEASY-T5 Zero Cloning Kit
        6. pEASYTM-Blunt Cloning Kit
        7. pEASY-Blunt Simple Cloning Kit
        8. pEASY-Blunt Zero Cloning Kit
        9. 配有Trans1-T1
    2. 2 转化
      1. 克隆感受态细胞
        1. Trans10 (Top10)
        2. Trans5α(DH5α)
        3. Trans109 (JM109)
        4. Trans110 (JM110)
        5. Trans1-Blue (XL1-Blue)
        6. Trans2-Blue (TL-Blue)
        7. Trans1-T1 (Mach1-T1)
        8. DMT Chemically Competent Cell
      2. 抗生素
        1. IPTG、 X-gal、 Ampicillin、Kanamycin
  9. 提取质粒
    1. 阳性克隆鉴定
      1. PCR、酶切鉴定
      2. 测序鉴定
    2. 质粒小提
      1. ArtMedia Plasmid Culture(高效培养基)
      2. EasyPure Plasmid MiniPrep Kit
  10. 后续相关实验
    1. 点突变
      1. Easy Mutagenesis System
      2. Fast Mutagenesis System
    2. 原核表达(需换原核表达载体)
    3. 真核表达(需换真核表达载体)