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Data analyses
- Microarray 12th vs SSP
- Solexa RnaSeq alignments, quantitation
- Gene annotations
- Microarray cross-species
- Avian cross-species CGH data
- GBrowse work enabling data analysis
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GBrowse, finch data, enhancements
- GMOD/GBrowse/Chado introduction
- GMOD/Chado architecture slide
- Songbird DB architecture (w/ Chado?)
- GBrowse content: finch genes, predictions, 454 ESTs, Budgie genome, xenoRefGene, Duke clones, oligoarray oligos
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GBrowse functions
- Text search -- using PostgreSQL full-text search heuristics
- BLAT/BLAST sequence alignments
- Feature-feature comparisons/overlaps/etc.
- Balloon hover content -- cross-links
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Interesting GBrowse views -- conclusions "seen"
- 454 ESTs and Duke clones suggest another exon
- Budgie genome alignment, mostly over the exons (also 454 ESTs)
- GBrowse search with multiple hits
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Gene annotations
- Exonerate clones to genome (intron boundary-aware)
- Download latest EnsEMBL gene coordinates
- Download latest xenoRefGene
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Describe heuristic used in latest annotations
- If Duke name/descr, use that
- Gene name majority -- xenoRefGene, finch EnsEMBL name, human/chicken BLAST, Duke name
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Budgie 454 genome sequence assembly
- estimating coverage by bases, contigs, exons, extended alignments